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Determination of Phenolic Compounds’ Extraction Conditions from Pistacia palaestina Leaves at Two Different Stages of Maturity

[ Vol. 16 , Issue. 5 ]

Author(s):

Wissam Zam*, Ali Ali and Razan Hasan   Pages 808 - 814 ( 7 )

Abstract:


Background: Phenolic compounds are distributed throughout the plant kingdom. Numerous investigations have proved that Pistacia species have secondary metabolites, and these contain different classes of bioactive phenolic compounds.

Objective: The objective of the present work was to optimize the extraction conditions of phenolic compounds from Pistacia palaestina leaves cultivated in Syria and find out the effects of solvent type, extraction time, and temperature on total phenolic content (TPC) and in vitro antioxidant activity% (AA%). Two different growth stages were studied.

Methods: Antioxidant activity and total phenolic content values were estimated using 2,2’-diphenyl-1-picrylhydrazyl and Folin-Ciocalteu methods respectively.

Results: The highest TPC and AA% were found in fresh leaves with 14.32 g/100g and 92.69% respectively. The optimum extraction solvent for phenolic recovery and antioxidant capacity was acetone 40%. Changes both in temperature (25, 40, 60 and 80 °C) and time (15, 30, 45 and 60 min) had no significant influence on TPC and AA% of Pistacia extracts.

Conclusion: Phenolic compounds could be extracted in a short time and low temperature and could be used as natural antioxidant agents.

Keywords:

Antioxidant activity, DPPH, leaves, maturity stages, Pistacia palaestina, total phenolic compounds.

Affiliation:

Department of Analytical and Food Chemistry, Faculty of Pharmacy, Al-Andalus University for Medical Sciences, Tartous, Department of Food Technology, Faculty of Technical Engineering, Tartous University, Tartous, Department of Food Technology, Faculty of Technical Engineering, Tartous University, Tartous



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