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Momordica charantia Seed and Aryl Extracts Potentiate Growth Inhibition and Apoptosis by Dual Blocking of PI3K/AKT and MAPK Pathways as a Downstream Target of EGFR Signaling in Breast Cancer Cells

[ Vol. 16 , Issue. 5 ]

Author(s):

Guzide Satir Basaran*, Hatice Bekci, Ayse Baldemir, Selen Ilgun and Ahmet Cumaoglu   Pages 726 - 733 ( 8 )

Abstract:


Background: Herbal extracts and plant compounds are increasingly being of interest for their therapeutic potential in various cancer types. Momordica charantia is well known for its anti-diabetic, anti-inflammatory, anti-cancer properties.

Objective: In the present study, we investigated the antiproliferative and pro-apoptotic effects of Momordica charantia seed and aryl extracts on Breast cancer cells and explored the underlying molecular mechanisms.

Results: Our results showed that both extract significantly inhibited the growth of MCF-7 and MDA MB-231 cells in a concentration dependent manner, and induced apoptosis by upregulation of caspase 9 and caspase 3 mRNA levels. In addition, in different incubation time, both extract evidently inhibited EGF induced EGFR phosphorylation/activation in both cell lines. Moreover, Momordica charantia aryl and seed extracts inhibited phosphorylation/activation of PI3K/AKT and MAPK (ERK and P38) pathways in both cell lines.

Conclusion: The current study clearly demonstrates that the Momordica charantia aryl and seed extracts have the potential to exert its cytotoxic effect on breast cancer cells by a mechanism involving inhibition of EGFR and EGRF releated pathways with induction of apoptosis. The overall finding demonstrate that this plant specially seed extract, could be potential source of new anticancer compounds for possible drug development against cancer.

Keywords:

Anticancer compounds, AKT, breast cancer, EGFR, MAPK, Momordica charantia.

Affiliation:

Department of Biochemistry, Faculty of Pharmacy, Erciyes University, Kayseri,, Department of Food Engineering, Faculty of Engineering, Erciyes University, Kayseri,, Department of Pharmaceutic Botanic, Faculty of Pharmacy, Erciyes University, Kayseri,, Department of Pharmaceutic Botanic, Faculty of Pharmacy, Erciyes University, Kayseri,, Department of Biochemistry, Faculty of Pharmacy, Erciyes University, Kayseri



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