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Caffeine Improves Hormonal and Lactate Homeostasis in Diabetic Rats


Luiz Augusto Silva*, Jessica Wouk, Vinicius Weber, Pablo Almeida, Julio Cesar Martins, Carlos Malfatti and Raul Osiecki   Pages 1 - 5 ( 5 )


Introduction: Lactate minimum test (LMT) identifies a sustainable exercise intensity, in which an equilibrium is observed between production and clearance of blood lactate and the hormone influence during this physiological moment. Objective: The present study aimed to identify the levels of LM and hormones after caffeine consumption and exercise stress test (ST) in diabetic rats. Material and Methods: This study was composed of 24 animals, of 60 days, allocated into four groups: Control, Diabetic, Caffeine, and Diabetes+Caffeine. The Diabetes model was induced by intraperitoneal administration of 120 mg/kg of alloxan. On the test day, 6 mg/kg of caffeine were administrated 30 minutes before the exercise stress test (ST) protocol. During the ST animals underwent a stress test (ST), in which they performed forced swimming (until exhaustion) tie to loads of 13% body’s weight (bw). The incremental phase of LM began with an initial load of 4% body’s weight (bw) and increased 0.5% every 5 min. Lactate concentration was measured 5, 7 and 9 min (mmol/L) after ST. The incremental progressive test (IPT) involved swimming with loads of 4.0, 4.5, 5.0, 5.5, 6.0, and 7.0% of bw, for 5min with each. Blood samples were collected by a caudal puncture to subsequent lactate and hormone assay. Results: Performance time and lactate concentration of hyperlactatemia test, as well as lactate minimum (LM) and lactate (LAC) concentration after the progressive test presented a significant difference when comparing the levels of the control group with caffeine and diabetic group (p<0.05). Conclusion: It is suggested that caffeine improves lactate clearance and hormonal steady state condition of diabetic animals after hyperlactacidemia and physical exercise maintenance.


Metabolism, Lactate, Conditioning


Guairaca College, Physical Education, Midwest State University, Post-graduation of Pharmaceutical Science, Midwest State University, Physical Education, Guairaca College, Physical Education, Guairaca College, Physical Education, Midwest State University, Physical Education, Federal University of Parana, Physical Education

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