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Acute and Subacute Toxicity Study on Dietary Supplementation with Soy Isoflavones in Wistar Rats

[ Vol. 14 , Issue. 1 ]

Author(s):

WCS Faria, RC Martinelli, AS Arcas, IF Da Silva Junior, EM Colodel, DFLC Cavenaghi, AP Oliveira and WM Barros   Pages 68 - 78 ( 11 )

Abstract:


Objective: The aim of this study was to investigate the dose-dependent toxic effect of SIF supplementation.

Methods: Single doses of 0, 100, 500, and 1000 mg/kg bw of SIF were administered by gastrogavage to male and female Wistar rats (6/sex/group). Same dosages were gastrogavaged daily over a period of 30 days, the animals were fed with a standard ration added of 20% soybean-based protein bar (weight/weight) ad libitum. Food consumption, Body weight, organ weights, hematological and biochemical parameters were measured, and then the organs were necropsied and examined histologically.

Results: A single dose of 1000 mg/kg bw did not cause any behavioral alteration, toxicity symptoms, or death. The SIF at 1000 mg/kg bw/day for 30 days exhibited increasing a relative liver weight in the female. In this same dosage, a decrease in relative liver, lungs, kidneys, spleen and heart weight was observed among males. A minimal alteration in enzymes or biochemical markers of liver function was observed in female and male rats, but this was not considered adverse since there were no supporting clinical or histopathologic findings.

Conclusion: The acute toxicity test indicates that SIF has a high margin of safety as shown by the LD50 and according to subacute test the No-observed-adverse-effect level (NOAEL) of SIF was estimated to be 100 mg/kg/day. However, the chronic toxicity cannot be estimated.

Keywords:

Acute toxicity, glycine max, phyto-oestrogen, rodents, subacute toxicity, supplementation.

Affiliation:

Faculty of Food Engineering, Department of Food Science, State University of Campinas, UNICAMP, University City Zeferino Vaz, Barao Geraldo, Campinas, SP, CEP 13083-970, Faculty of Food Engineering, Federal Institute of Education, Science and Technology of Mato Grosso, IFMT. Juliano Costa Marques Street, Bela Vista, Cuiaba, MT, Faculty of Food Engineering, Federal Institute of Education, Science and Technology of Mato Grosso, IFMT. Juliano Costa Marques Street, Bela Vista, Cuiaba, MT, Laboratory of Pharmacology, Department of Basic Sciences of health, Faculty of Medicine, Federal University of Mato Grosso, UFMT, Fernando Correa da Costa Street, nº 2367, Boa Esperanca, Cuiaba, MT, Laboratory of Veterinary Pathology, Department of Medical Clinic Veterinary, Faculty of Veterinary Medicine, Federal University of Mato Grosso, UFMT, Fernando Correa da Costa Street, nº 2367, Boa Esperanca, Cuiaba, MT, Faculty of Food Engineering, Federal Institute of Education, Science and Technology of Mato Grosso, IFMT. Juliano Costa Marques Street, Bela Vista, Cuiaba, MT, Faculty of Food Engineering, Federal Institute of Education, Science and Technology of Mato Grosso, IFMT. Juliano Costa Marques Street, Bela Vista, Cuiaba, MT, Faculty of Food Engineering, Federal Institute of Education, Science and Technology of Mato Grosso, IFMT. Juliano Costa Marques Street, Bela Vista, Cuiaba, MT

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