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Calorie Restriction Attenuates the Age-Associated Decrease in Subunit of F1 ATP Synthase Activity in the Mouse Skeletal Muscles

[ Vol. 12 , Issue. 4 ]


Chandan Kumar Jana and Nilanjana Das   Pages 236 - 240 ( 5 )


Objective: In the present investigation, the effect of a long-term caloric restriction (CR) on the age-associated decrease in enzymatic activities of the mitochondrial α subunit of F1 ATP synthase in the mouse skeletal muscle was studied in a strain known to exhibit CR-mediated extension of life spans.

Design and Method: Animals were randomly assigned to either control or CR groups and fed on an ad libitum or 60% of the food consumed by the control group, respectively. The CR group diet was enriched in vitamin content in order to balance the micronutrient intake between the groups. After 18-months on these diets, simple enzymatic assays were used for analyzing the status of enzymatic activity of ATPase in both the control as well as CR animals (n=8 from adult AL control and CR groups, respectively, each animal being treated separately).

Results: It was observed that a CR-diet regime on C57BL/6Nnia strain of mice resulted in significant augmentation of activity (18.8%) of the mitochondrial subunit of F1 ATP synthase with respect to the age-matched controls animals on an ad libitum diet.

Conclusion: Since skeletal muscle is a tissue whose activity is dependent on an abundant supply of ATP, our experiments indicate a possible causal role of CR in the observed healthier lifespan and better muscular coordination of animals on CR diet regime than the ad libitum controls.


Calorie restriction, ATPase, ATP synthase, aging, skeletal muscle, mitochondrial matrix, enzymatic activity, attenuation.


Department of Biotechnology, Visva-Bharati University, Santiniketan, West Bengal 731 235, India.

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